ChIP-chip and ChIP-seq

Chromatin immunoprecipitation (ChIP) is an in vivo method of identifying the bonding sites of proteins to a DNA. This approach can be applied to analyze the interaction of transcription factors to the target cis-elements on the genome or the possible modification of histones. The combination of ChIP with array (ChIP-on-chip) or next generation sequencing (ChIP-seq) will provide a genome wide analysis of protein-DNA interaction and histon modifications. The knowledge about such interactions and modification is important for the understanding of many biological processes and various diseases.

We offer both chip and sequencing service as a complement to you ChIP experiment.
Non-coding RNAs

The availability of next generation sequencing provide a unique opportunity for scientists to analyze small non-coding RNAs like miRNAs (micro RNAs), siRNAs (short interfering RNAs) or piRNAs (piwi-interacting RNAs) or other classes of untranslated transcripts in a time and cost efficient fashion. Non-coding RNAs are functionally important RNAs that implement into various functions in the regulation of cellular processes. They play a central role in regulating gene expression and are subdivided into different classes by function and size. Depending on the aim of the project differently sized libraries can be prepared to analyze different classes of untranslated transcripts.

DNA methylation

The analyze of DNA methylation is also very important to understand transcriptional control. High-throughput Next Generation technologies now allow genome-scale mapping of these modifications. Several large-scale analysis techniques are available that enable the survey of DNA methylation status at nucleotide resolution throughout the genome including Next Generation Sequencing coupled with bisulphite treatment of DNA.

For more information please contact: